PCR stands for ______________________; and is used for _______________________________.
A. polypeptide cyclical reaction; directly identifying the sequence of DNA
B. polymerase cancer reaction; making copies of cells by stimulating mitosis
C. polymerase chain reaction; making copies of DNA
D. polycyclic carbon repeats; mutating DNA to identify criminals
In a PCR reaction, DNA must be denatured. Which option below best describes that step-
High temperatures are used to break covalent bonds of DNA to create huge numbers of free-floating nucleotides
High temperatures are used to disrupt hydrogen bonds between DNA strands to separate the strands
Very low temperatures cause the DNA to change shape and replicate
Moderate temperatures promote RNA polymerase to copy the DNA into RNA
3. What is a primer and why is it used in PCR?
A. A primer is a short, specific piece of single-stranded DNA that provides a starting point for the enzyme that will do the copying
B. A primer is the enzyme that will do the rapid copying of PCR
C. A primer is a short repeated sequence that is being looked for during the PCR
True or False
______ 4. STRs stands for “Short Tandem Repeats”
______ 5. Most of your genome, (over 50%) is unique to you.
______6. In this lab we are using PCR to amplify a region of the genome known to contain a STR.
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