1.   In your research you are analyzing the changes in
live gene expression in a mouse model of nonalcoholic fatty liver
disease. You previously performed microarray analysis on livers of
mice given a high-fat diet verses mice on regular diet and found a
number of genes that were abnormally expressed in the high-fat diet
mice. You perform quantitative RT-PCR on some of these genes and
receive the following CT values:

GAPdh   Cyp8b1      
Cyp17a1   Asns       
Past1
High-Fat Diet   10 27 25 21 23
Regular Diet 11 25 21 26 30

A.   For each of the genes, complete calculations
using the delta-delta Ct method to determine the relative fold
change in gene expression for the mice with the high-fat diet.
(Hint: use GAPdh as the reference gene) show your work. (8pts)