1. Provide a hypothetical example of a DNA sequence that might
be recognized by a restriction endonuclease. Explain how this is
different from most DNA sequences. How would the cleavage site
within your sequence determine if the products had sticky or blunt
ends?

2. What are important elements of the pBR322 plasmid vector (see
image below) that make it useful in cloning foreign pieces of DNA?
Why is each important? How would you screen to find a bacterium
that contained the pBR322 plasmid with a piece of foreign DNA
inserted into the EcoRV site?

3. What are the relative positions and different roles of the three
tRNA binding sites on the actively translating ribosome?