11. Below is an agarose gel electrophoresis result from a reverse transcriptase PCR experiment. Lanes 1 and 6 are molecular weight markers, and lanes 2-5 are the results from RT-PCR reactions anlyzing the tissues listed below Lane l and 6: Molecular weight standard band sizes are 2176, 1766, 1230, 1033, 653, 517, 453, 394, 298, 234-220, 154 bp Lane 2: newborn lver tissue biopsy Lane 3: 6 month fetal liver Lane 4: 3 month fetal liver Lane 5: 3 week embryo (all tissues) 1 234 5 6 Fully interpret the result with respect to- a) Which tissues are expressing the gene? Can you tell? b) Which tissue is expressing the gene at the highest level? And at lower levels? Can you tell? c) What is the size of the mRNA in nucleotides? Can you tell? 12. Consider the northern blot data below kb) 9.49→ 7.46→ 4.40→ 2.37 1.35 0.24 A. Carelully examine the lane labeled “Kidncy” and the lane labeled “Spleen.” What can you conclude from comparing thc results in these two lanes? B. Carefully examine the lane labeled “Spleen” and the lane labeled “Testes.” What can you conclude from comparing the results in these two lanes?