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Question: 12. (6 points) While setting up your restriction digest, you realize that you used the wrong NEBu…

by | Dec 4, 2023 | Posted Questions

Question: 12. (6 points) While setting up your restriction digest, you realize that you used the wrong NEBu...

12. (6 points) While setting up your restriction digest, you realize that you used the wrong NEBuffer. You were supposed to use buffer 1 and instead you used buffer 3. You look up the activity of your enzyme in buffer 3 and find that it is not at all active (so your digest is doomed). Worse, you had used all of the last bit of plasmid DNA to set up the digest, so you can’t simply throw this away and set up another one. You’ll have to go back and make more plasmid DNA first before you can do the digest unless you can think of a way to salvage this experiment. What might you be able to do to correct your mistake (rescue your DNA and get it into the correct buffer)?

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