2. (25 pts) An ester is hydrolyzed by immobilized chymotrypsin at a maximum overall volumetric rate of 18.4 umol ester per cm3 per s and a Michaelis constant for the substrate of 200 . The effective diffusion coefficient of the ester has been estimated to be 4×10″ cm” per s. Determine the range of biocatalyst sphere radii appropriate for studying the immobilized enzyme kinetic properties under reaction- limited conditions.