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Question: 2.2a) A preparation of reduced and carboxymethylated Mouse-Elberfeld virus protein particles in w…

by | Dec 5, 2023 | Posted Questions

2.2a) A preparation of reduced and carboxymethylated
Mouse-Elberfeld virus protein particles in water reached
sedimentation equilibrium at 25OC after 40 hr at 12,590 rpm. The
following data show the recorder displacement (proportional to
concentration) versus r for this protein:?

C (arbitraryunits) 2.29 2.51 2.79 3.09 3.51 3.89 4.47 5.01 5.89
6.61 7.41 8.51

r (cm) 6.55 6.58 6.60 6.65 6.67 6.69 6.71 6.74 6.76 6.79 6.81
6.84

Use these results to evaluate the mass of the particles present
(Rho protein = 1.370 g cm-3) and estimate Ro
and fo for the molecules. Does the sample appear to be
monodisperse?

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