Consider the following enzyme-catalyzed reversible reaction: E
<–> M, which is energetically unfavorable (ΔG0 = 4.3
kcal/mol). In a typical bacteria at 37°C, the enzymatic reaction is
coupled to hydrolysis of ATP, which provides -10kcal/mol. How much
additional energy is required to drive the reaction to the same
equilibrium concentrations observed at 37°C in a thermophilic
organism, which lives in the hot springs at Yellowstone National
Park at 125°C? Remember that K = [M]/[E], ΔG0 = -RTlnK, and R =
1.987×10-3 kcal/mol K.