DO NOT USE COMPUTER GENERATED GRAPHS
!!!!!!!!!!!!!!!!!!!!!!!!!
The following kinetic data were obtained for an enzyme in the
absence of inhibitor (1), and in the presence of
two
different inhibitors (2) and
(3) at 5 mM concentration. Assume [Etotal] is the
same in each experiment.
| [S] (mM) |
1 ν(μmol/mL. sec) |
2 ν(μmol/mL. sec) |
3 ν(μmol/mL. sec) |
| 1 | 12 | 4.3 | 5.5 |
| 2 | 20 | 8 | 9 |
| 4 | 29 | 14 | 13 |
| 5 | 35 | 21 | 16 |
| 12 | 40 | 26 | 18 |
DO NOT USE COMPUTER GENERATED GRAPHS
a. From a double-reciprocal plot of the data, Determine Vmax and
Km.
b. Determine the type of inhibition that has occurred in 2 and
3.
DO NOT USE COMPUTER GENERATED GRAPHS! ANSWERS MUST BE HAND
WRITTEN
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