Endsem_developmental biology_please answer only
question no.6_1 mark

6. You are trying to map the enhancer sequences for a
gene that is normally expressed in the brain of organism X. you
generated a series of constructs (spanning different lengths of the
gene region) and tagged with reporter gene GFP to study the
expression pattern. Interestingly for one of the constructs, the
GFP expression became ubiquitous and was expressed in all the all
the cells of the body of the organism X. Analysis of the DNA
sequence indicated loss of two hundred and fifty bases from the
native configuration. The lost DNA in the truncated construct most
probably contained
A. Enhancer
B. promoter
C. Silencer
D. Transcription terminator
E. Translation terminator .