Answer these please 🙂 DO NOT ANSWER IF YOU CAN NOT ANSWER
ALL.
Figure 1. 3. We will quantify your product using spectrophotometry a. Write out Beer’s Law below b. The quantification of DNA is so common that researchers often use a short cut. An absorbance of 1.0 at 260 nm, with a 1-mm pathlength, is approximately 50 ng/ul What is the concentration of a sample, if the A2so is measured at 0.75? 4. To prepare for sequencing, you will need to dilute your sample to 25 ng/ul in a final volume of 10 μ1. a. How many ng total will you submit for sequencing? b. Given the concentration from Question 3b, how many microliters of sample will you combine with water to prepare your sample for submission? P-17





