For this question, please use the information provided in the table below: Free- Energy Changes of Glycolytic Reactions Glycolytic reaction step AG’° (K/mol) 16.7 1.7 14.2 23.8 7.5 6.3 18.8 4.4 7.5 10 31.4 A. What would happen to a reaction if the mass action ratio, under cellular conditions, is larger than the equilibrium constant, Keq? Why are reaction steps that are far from equilibrium good control points in metabolic pathways? 4 sentences max. B. Explain why Pi (inorganic phosphate) is absolutely required for glycolysis to proceed all the way to pyruvate production. 3 sentences max. C. The standard free-energy change for Step 4 in the forward direction written is +23.8 kJ/molThe concentrations of the three intermediates in the hepatocyte of a mammal are: fructose 1,6- bisphosphate, 1.4 x 105 M ; glyceraldehyde 3-phosphate, 3 x 10® M; and dihydroxyacetone phosphate, 1.6x 10$ M. At body temperature (37°C), what is the actual free-energy change for the reaction? D. According to this chart, Steps 1, 3, 7, and 10 are irreversible in glycolysis (large negative AG®) However, in reality, there are only 3 irreversible steps. Which of the aforementioned steps is reversible? Explain this observation. 4 sentences max. E. How does the rate of glycolysis change if there is an overabundance of ADP in the cytosol? which steps and enzymes would be affected and how? 4 sentences max.