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Question: Hydrophobic interaction chromatography exploits the interaction between hydrophobic resin and hyd…

by | Dec 3, 2023 | Posted Questions

Hydrophobic interaction chromatography exploits the interaction
between hydrophobic resin and hydrophobic sidechains of peptides
for separation. For the peptide with sequence NFPDDDYKDEHF, what pH
would you predict for this peptide to be retained on the HPLC
column most strongly? (Hint: is this peptide acidic or basic?)
Please explain your answer

a. pH = 3

b. pH = 6

c. pH = 11

d. Cannot determine from these data.

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