ICE#5: Prokaryotic Transcription Unit-Initiation & Rho-independent Termination Show your work below. 6 pts Student name Section #:- 1. (2 pts) Draw & Label a bacterial DNA transcription unit using all the following: 40, -35 and -10 consensus sites terminator which is within the RNA-coding region and transcription start site at +1 RNA-coding region has 2 consensus sequences Inverted Repeats (IR) * polyA DNA run *non-template & template strands “strand polarity upstream and downstream 2. (4 pts) [use the back of paper if necessary] a. Transcription initiation: explain where the sigma factor will bind and why that helps initiation. b. Transcription termination: draw it. Draw (on the back of the paper) the DNA-RNA hybrid at the 3′ end: label the DNA polyA, the mRNA polyU and the hairpin in the RNA. c. Indicate what structure makes RNA polymerase pause at 3′ end: d. In the rho-independent termination setting, explain why a helicase is NOT needed for the mRNA to separate from the DNA: