In a denaturing PAGE, a mix protein sample including following proteins was loaded a. How many bands will be shown on gel b. Draw a slab gel to show the bands as a results of electrophoresis of proteins. a c. Label the bands (pay attention to the order of separation) 1 Egg white albumin (Mw:45.000 D subunit ac 10, 500D and subunit bc 34, 5000) 2 Serum albumin(MW 65,000 D) 3-Lysozyme (MW: 13,000 D) 4 Chymotrypsin (Mw: 21,000 D) If you load a mix of Dative and denatured sample of a protein subunit in one single well bow many bands you will detect and what would be the order of resolution from tope to the booms of gel Define the upper limit of temperature for enzyme assay. what would happen if you increase temperature above that limit? Why you have to keep all the solutions require for enzyme reaction such as enzyme, substrate, and buffers on ice before addition of the enzyme and incubation in desired temperature, In any enzyme reaction, Michaels Manton constant is the of at