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Question: In the following example regarding gene order via co-transformation frequnecy I amhaving a hard t…

by | Dec 5, 2023 | Posted Questions

In the following example regarding gene order via
co-transformation frequnecy I amhaving a hard time understanding
what the information actually means. I realize higher frequencies
result in closer linkage and closer distance between alleles,
however, I do not understand what the signifcance/relation is
between the observation of two different versions. For example, it
is my understanding that the percentages 3.00 and 0 are
co-transformation frequencies between his and leu, how can one of
them be 3 and the other be zero? is that not contradictory to
linkage?

donor : his+ leu+ lac+ recepient: his-leu- lac-

Genotype of transformed cells percentage of all cells
his+ leu+ lac+ 0.02
his+ leu+ lac- 0*
his+ leu- lac+ 2.00
his+ leu- lac- 4.00
his- leu+ lac+ .10
his- leu- lac+ 3.00*
his- leu + lac – 1.50
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