****Intro to Biotech****
The answers shouldn’t be longer than a sentence or two!

uestion 2 (50 points The figure below shows an unknown template strand with a primer attached to the starting sequence. A DNA sequencing reaction was conducted on the template strand in a thin capillary gel tube containing primers, DNA polymerase, deoxyribunecleotides and fluorescently tagged dideoxyribunecleotides and a computer was used to record the fluorescent peaks during the reaction’ TGCCGAAGC ACGGCTTC Primer: Template: G A TC 10 Time (s) Label the ends of the primer and template strand and show the sequencing direction unknown sequence and label the ends. How many fragment sizes were sequenced? fragment with a dash on the agarose gel and read the sequence and label the ends. mRNA samples from three organs labeled A, B and C. Describe how you can identify the a. b. If after 10 minutes, the fluorescent peaks are shown in the figure above. Determine the c. Assuming agarose gel electrophoresis was conducted on the fragments, indicate each d. Assuming the sequence is a gene expressed by an organ and you have three minute organ that is expressing the gene e. Describe how you can use gene micro array to determine if an unknown DNA sample contains a gene involved in causing a known disease





