Case study help!
LAB #13 USING DNA TO IDENTIFY MICROORGANISMS final Microbiology lab you must determine what infectious agent each For youri infected with by using all of the knowledge you have acquired semester. Initially. read through the case studies and fill in all of confirm your prognosis, run the DNA patient is the information you can on the chart. To on an agarose gel and compare the gel to your control. Patient #1 r very sick friend with blood-tinged sputum coughed on patient #16 months ago. For the past 3 weeks, patient #1 has been complaining of a chronic cough, weight loss, fever and fatigue. The causative agent is an obligate aerobe and is very slow growing. A type IV delayed hypersensitivity response can be seen with this patient that is typical of a To immune response. A toxin found in the bacteria can to antibodies. The DNA sample was amplified to detectable bind levels and cut with the appropriate restriction enzyme. Patient #2 After eating uncooked hamburger meat, patient #2 became very sick with diarrhea and nausea. The causative ogent is facultatively anaerobic. Antibodies were unable to bind to the toxins produced by this organism. The patient exhibited a nonspecific immune response. The DNA sample was amplified to detectable levels and cut with the appropriate restriction enzyme. Patient #3 Patient #3 cut his foot on a shell at the beach. He then complained of swelling, pain and redness in the foot the following day. The toxins produced by this organism did bind to antibodies and caused a very specific response in the patient. IgG levels were increased. The DNA sample was amplified to detectable levels and cut with the appropriate restriction enzyme. Patient#4 Patient #4 spent time taking care of 2 friends that were sick. One of the rriends was diagnosed with influenza virus and the other friend was





