MCQ 16 TTC PUCs Snal EcoRI Modern plasmid vectors have features typically as illustrated in this map of pUC18. A tandem array of restriction enzymes is positioned in a so-called multicloning site (MCS). What would be different about a DNA fragment ligated into the Smal site in one reaction, and into a double site such as Smal-EcoRI? (A) The insert will be ligated in one direction only in the Smal site (B) No real difference, the insert will have been successfully ligated (C) The insert will be ligated in one direction only in the Smal-EcoRI site. (D) The insert will be ligated in either direction in the Smal-EcoRi site. (E) The insert will be ligated in the same direction in both ligations. MCQ 17 The two sequences shown below are palindromes for restriction enzymes that make blunt-ended cuts as shown by the arrows. If composite hybrid sites were ligated from these blunt cuts, the new sites would be.. (A) The same as the original sites. (B)- Different to either of the original sites and unable to be cut with either enzyme. (C-Different to either of the original sites and able to be cut with one but not both enzymes. (D)-Different to either ofthe original sites and able to be cut with both enzymes. (E)-Composites of the original sites and able to be cut with both enzymes.