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Question: Need to identify an unknown sample that was distributed and kept in
an inoculating chamber
at 30 …
Need to identify an unknown sample that was distributed and kept in
an inoculating chamber
at 30 degrees celsius.
These are the results from the tests conducted…
Colony morphology:
•Entire = Filamentous
•Edge= Rhizoid
•Elevation= Umbonate
______________________
Gram stain= Gram +
______________________
Carbohydrate Fermentation:
•Glucose= +
•Sucrose= –
•Lactose= –
(all in phenol red broth)
______________________
Starch Hydrolysis= +
Citrate Hydrolysis= –
EMB Growrh= none
MR and VP tests= both negative
Oxidase= –
Catalase= –
Casein Hydrolysis= +
Gelatin Hydrolysis= +
Indole= –
Phenylalanine Deaminase= –
Bile Esculin Agar= +
Direct observed motility= –
________________________
After onsverving that it was a gram positive rod, I determined
it was of the bacillus genus. The following bacillus bacteria we
could have are:
•Bacillus cereus
•Bacillus circulans
•Bacillus coagulans
•Bacillus megaterium
•Bacillus subtilis
So far I’m leaning more towards Bacillus cereus, but any
additional help would be appreciated. Also included is a photo of
the gram stain, broth tube, and also a photo of the colony
morphology
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