Questions cMe from GENETICS LAB

Will you help me solve these problems.. the questions are from
GENETICS labs 8-9

words such as did mom, and pop have something in common with the fundamental tool of recombinant DNA technology In the context of recombinant DNA technology, which would be used to describe such words? term A) lysogenic B) prototrophic C) palindromic D) conjugation E) insertion 2) Assume that a plasmid (circular) is 3200 base pairs in length and has restriction sites at the following locations: 400, 700, 1400. 2600. Give the expected sizes of the restriction fragments following complete digestion. A) 400, 800, 1000 (2 of these) B) 400, 1200, 1600 CO 300, 700, 2200 D) 700, 400, 1400, 2600 E) 300, 700, 1000, 1200 3) Assume that you have cut DNA with the restriction enzyme HindlII. You separate the fragments on an agarose gel and stain the DNA with ethidium bromide. You notice that the intensity of the stain is less in the bands that have migrated closer to the pole. What explanation below is not correct for this finding? A) the smaller fragments bind less stain B) the smaller fragments migrate toward the pole C) the smaller fragments migrate more away from the origin than larger fragments which are near the origin D) the larger fragment stains more than smaller fragment 4) List two especially useful characteristics of cloning vect A) high copy number and antibiotic resistance gene(s) B) virulence and lysogenicity lytic cycle C) ability to integrate into the host chromosome and then causing a D) nonautonomous replication and transposition E) reverse transcriptase and ligase activities 5) over the years, sophisticated plasmid vectors have been developed d for use in recombinant DNA technology. Point out the feature below that has not been introduced in particularly us vectors. inserts A) small size to allow large B) low copy number cloning sites) large numbers of unique restriction sites (multiple antibiotic resistance) D) variety of selection schemes (pigmented colonies,