Sequencing using massively parallel next generation sequencing
. Sequencing using massively parallel next generation sequencing You are tasked with sequencing the gene for tyrosinase, an enzyme important in the synthesis of melanin and used in detection of melanoma. You decide to use next generation sequencing (NGS). Below is the output of NGS from three fragments of the gene. Each box is a sequential time point of the sequencing process moving left to right. So that you can see it in black and white, to represent each nucleotide shapes are given rather than different fluorescent dyes. The what is the read sequence for the three fragments? Be sure to label the 3′ and 5′ ends. Determine the sequence of the complete nucleotide IEMPLATE sequence (hint: to make this sequence alignment, know there is a 4 nucleotide overlap for each fragment.) A)





