Supercoiling is an important property of DNA tertiary structure that affects essential processes such as replication and transcription How do cells maintain DNA in a negatively supercoiled state? OOnly protein free closed-circle DNA molecules are able to maintain a negatively supercoiled state. O Only nicked, circular molecules of DNA can maintain a negatively supercoiled state To maintain a negatively supercoiled state, the DNA molecule needs to either be a closed circle or if linear, constrained at both ends by proteins. Only linear molecules of DNA can maintain a negatively supercoiled state. Which structural changes become more favorable when a DNA molecule is negatively supercoiled? DNA triple helix formation cruciform formation formation of Z-form DNA undefined formation of A-form DNA □ □