Suppose you wish to determine if the small subunit of the
Na+K+ATPase is a transmembrane protein, with domains that extend
out from the membrane on both sides. Design an experiment using a
protease and cultured neurons to determine if this is the case.
What result would you expect if the protein only extends out on the
external side of the membrane. How would you do this with gel
electrophoresis and western blotting





