This is just a reference video shows the basic idea of what
happens during Excersice 12.2

Exercise 12.2 Agarose gel electrophoresis

1)Transfer 18 μL of pPCR850
plasmid to a new sterile microcentrifuge tube.

2)Transfer 2 μL of EcoRI-HF
RE-Mix to the microcentrifuge tube containing pGEM-pcr850.
Mix thoroughly.

3)Transfer 10 uL of pGLO
plasmid to a new sterile microcentrifuge tube.

4)Transfer 10 μL of HindIII-HF
RE-Mix to the microcentrifuge tube containing pGLO. Mix
thoroughly.

5)Incubate both tubes in a
37^oC water bath for 30 – 60 min.

6)While the plasmids digest:

a) Use the new spectrophotometers to analyze the genomic
DNA samples  

b) Set-up for the electrophoresis chamber.

8) Prepare two negative control by
combine
a) 5 μL of the undigested pPCR850 plasmid with 2 μL loading
dye

b) 5 μL of the undigested pGLO
plasmid with 2 μL loading dye

9) Well #1 – Load 5
μL of the DNA ladder

10) Well #2 – Load 7
μL of undigested pGLO (from step
8b)

11) Well #3 – Load 20
μL of HindIII digested pGLO (from
step 4)

12) Well #4 – Load 7
μL of undigested pPCR850 (from
step 8a)

13) Well #5 – Load 20
μL of EcoRI digested pPCR850 (from
step 2)

Questions: