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Question: Using the technique demonstrated by your TA, remove 3 microcentrifuge tubes (1 5 mL capacity) fro…

by | Dec 5, 2023 | Posted Questions

Question: Using the technique demonstrated by your TA, remove 3 microcentrifuge tubes (1 5 mL capacity) fro...

Using the technique demonstrated by your TA, remove 3 microcentrifuge tubes (1 5 mL capacity) from the sterile container on your lab bench. With the micropipettors, deliver 10 mu L of water to one, 100 mu L to the second, and 1000 mu L (1 m L) to the third. As you pipet the water, take note of the level it reaches in the tip. After dispensing the water, compare the level of liquid in the tubes. Would you be able to recognize an inaccurate volume drawn into the tip or delivered to the tube if your pipetting technique was not particularly good? Discard the water in the 3 microcentrifuge tubes, and pipet 5 mu L in triplicate. Compare the levels in the tubes. Do you agree that the volumes are identical? Repeat with 50 mu L and 500 mu L. Is your technique reproducible? Make a 10X-dilution series of your assigned food coloring in water in the three empty microcentrifuge tubes, starting with 1 rightarrow 10. Prepare 100 mu L for each sample. Mix by closing the tube and placing it briefly on the vortexer. In the following table (Question #7), list the volumes of water and food coloring you will combine to accomplish this. Again, using the blue food coloring as an example, calculate and fill in the final dilutions of food coloring and the concentrations of dye that will result. Complete the following table, being mindful of significant digits: Compare the final concentration of dye generated in the 2X dilution series in Question #6 and the final concentration generated in the 10X series in Question #7. H been necessary in the 2X series to achieve approximately the same final concentration achieved with three tubes in the 10X series? Comment briefly on how this may influence assays.

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