Why were there less colonies on the + transform Nutrient Agar +
amp plate than on the + transform Nutrient Agar plate when cells
were plated from the same microtube of cells? A.The uptake rate of
the plasmid varies. Not all the bacteria were transformed with
enough copies of the ampicillin resistance plasmid to neutralize
the ampicillin. B.All of the answer choices are correct.
C.Transformation is not very efficient. Many cells did not take up
the ampicillin resistance plasmid. D.The colonies closer to the
antibiotic discs were killed, while the farther away colonies
survived.