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Question: Write the sequences of 2 oligonucleotide primers that can be used to PCR amplify the entire DNA s…

by | Dec 4, 2023 | Posted Questions

Write the sequences of 2 oligonucleotide primers that can be
used to PCR amplify the entire DNA sequence shown below. Only one
of the two strands of the DNA segment is shown, reading 5′ to 3′.
Indicate the 5′ and 3′ ends of your oligonucleotides.

5′
-GTTCCAGTAGTTGTGTCGCCCGAGACTTCTAGCCCAGGTGGTCTTTCCGGTACGAGTTGGTCCGCAGATG

TAGAGGCTGGGGTGTCTGACCCCAGTCCTTCCCTCATCCTGGTTAGATCGGCCATCCACTCAAGGTCAGATT  -3′

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