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Question: Yeast are single-celled eukaryotic organisms that grow in culture as either haploids or diploids….

by | Dec 4, 2023 | Posted Questions

Yeast are single-celled eukaryotic organisms that grow in
culture as either haploids or diploids. Diploid yeast are generated
when two haploid strains fuse together. Seven haploid strains of
yeast exhibit similar growth habit: at 25 ∘C, each strain grows
normally, but at 37 ∘C, they show different growth capabilities.
The table below displays the growth pattern.

Question: Yeast are single-celled eukaryotic organisms that grow inculture as either haploids or diploids....

Part A

Describe the nature of the mutation affecting each of these
mutant yeast strains. Explain why stains B and G display different
growth habit at 37 ∘C than the other stains.

All the mutants are temperature –sensitive
mutants, carrying a mutant allele of a gene
required for normal growth at 37 ∘C. The typical mechanistic
explanation of this is that the gene is required for growth at
all temperatures, and the mutant
allele is functional at 25 ∘C but not 37 ∘C. This explains the
five mutants that cannot grow at 37 ∘C. For the
two mutants that grow slowly at 37 ∘C, the alleles
probably retain partial function at 37 ∘C.

Each of the mutant pairs of haploid yeast is fused, and the
resulting diploids are tested for their ability to grow at 37 ∘C.
The results of the growth experiment are shown below.

Question: Yeast are single-celled eukaryotic organisms that grow inculture as either haploids or diploids....

How many different genes are mutated among these seven yeast
strains?

Please try to explain the reasoning.

37°C growth data Strain A B C DEF G A O

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