You have been tasked with isolating two different proteins from a bacterium that grows at 37°C. Part I (a) The first protein you are isolating is found in the organelle of the bacterium that has a pH of 9.4 at 37°C. You need to make up a 1.0 M solution. Which of the following buffer(s) would you choose and why? (2 pts) (Hint: Please note that species concentrations are dependent on the temperature of the solution i e pKa is temperature dependent) Table Buffer Full Chemical Name MW pKa at pKa at (g mol 250C 37C MES 2-(N-Morpholino)ethanesulfonic acid hydrate 195.24 6.10 5.97 Bis-Tris Bis(2-hydroxyethyl methyl methane 209.25 6.50 6.36 302.37 6.76 6.66 PIPES 1,4-P razinediethanesulfonic acid HEPES eth piperazine-1-ethanesulfonic acid 238.30 7.48 7.13 TAPS [(2-Hydroxy-1,lbis methyl)ethyl)amino]-1- 243.28 8.40 8.18 bis(hydroxy propanesulfonic acid CHES 2-(Cyclohexylamino)ethanesulfonic acid 207.29 9.49 9.36 CAPS 3-(Cyclohexylamino)-1-propanesulfonic acid 221.32 10.40 10.02 Here are the structures for the above compounds Bis-Tris MES PIPES HO3S SO3H S-OH xH2O OHHO TAPS HEPES CHES HO SO3H S-OH HO OH CAPS NJ S-OH