You want to denature and renature proteins from spinach. You
have already blended the spinach into a green liquid. Your initial
attempts to heat and then cool the liquid end result in a gooey
semi-solid mess.
a) What is the semi-solid mess?
b) Remembering the ribonuclease experiments discussed during
lecture, you change 2 things about your experiment, then find
success. What did you change?
c) Would adding chaperones to the mess have helped? Why or why
not?





